Клонирование и анализ генов легких цепей иммуноглобулинов стерляди
Ýòè
äàííûå
óêàçûâàþò
íà òî, ÷òî
ïåðåõîä îò
êëàñòåðíîé
ê
ñåãìåíòàðíîé
îðãàíèçàöèè
ïðîèçîøåë â
ôèëîãåíåçå
ïîçâîíî÷íûõ
î÷åíü ðàíî,
âîçìîæíî
óæå ó
äðåâíèõ
ïåðâè÷íî-êîñòíûõ.
ÂÛÂÎÄÛ
1.
Ñêîíñòðóèðîâàíà
áèáëèîòåêà
êÄÍÊ ëåéêîöèòîâ
ñòåðëÿäè
ïðåäñòàâèòåëüíîñòüþ
6õ106
íåçàâèñèìûõ
êëîíîâ. ÏÖÐ
ÄÍÊ
áèáëèîòåêè ñ
ïîìîùüþ
âûðîæäåííîãî
ïðàéìåðà,
ñîîòâåñòâóþùåãî
J ñåãìåíòó
ãåíîâ ÈÃ,
ïîçâîëèë âûÿâèòü
ôðàãìåíò ÄÍÊ
äëèíîé 370 ïí,
ãîìîëîãè÷íûé
V ãåíàì ÈÃ
ïîçâîíî÷íûõ.
2.
Èç
áèáëèîòåêè
êÄÍÊ ñ
èñïîëüçîâàíèåì
ïðîäóêòà ÏÖÐ
â êà÷åñòâå
çîíäà
âûäåëåíî
ïÿòü êëîíîâ,
ñîäåðæàùèõ
âñòàâêè
êÄÍÊ ãåíîâ
L-öåïåé ÈÃ.
Ñðàâíèòåëüíûé
àíàëèç
ïåðâè÷íîé ñòðóêòóðû
êÄÍÊ êëîíîâ
ïîêàçàë, ÷òî
îíè ÿâëÿþòñÿ
÷ëåíàìè
îäíîãî
ñåìåéñòâà
ãåíîâ è
èìåþò
íàèáîëüøåå
ñõîäñòâî ñ
ãåíàìè êàïïà
òèïà.
3.
Ñîãëàñíî
ðåçóëüòàì
ãåíîìíîãî
áëîò-àíàëèçà
èäåíòèôèöèðîâàííîå
ñåìåéñòâî
ñîäåðæèò
íåñêîëüêî
äåñÿòêîâ V
ãåíîâ è
òîëüêî 2-4 Ñ
ãåíà, ÷òî
óêàçûâàåò
íà
ñåãìåíòàðíóþ
ôîðìó åãî
îðãàíèçàöèè.
4.
Ïîëó÷åííûå
äàííûå
óêàçûâàþò
÷òî ïåðåõîä
îò
êëàñòåðíîãî
òèïà
îðãàíèçàöèè
ãåíîâ ÈÃ,
õàðàêòåðíîãî
äëÿ õðÿùåâûõ
ðûá, ê ñåãìåíòàðíîìó,
ïðèñóòñòâóþùåìó
ó âñåõ ìëåêîïèòàþùèõ,
ïðîèçîøåë â
ýâîëþöèè â
ïåðèîä ïîÿâëåíèÿ
ïåðâè÷íî-êîñòíûõ
ðûá.
ÑÏÈÑÎÊ
ËÈÒÅÐÀÒÓÐÛ
Ìàçèí
À. Â.,
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Ê. Ä. è äð.
Ìåòîäû ìîëåêóëÿðíîé
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1988. 333 ñ.
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